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Лаборатория генной инженерии

Публикации

  1. Irina Lyapina, Anna Filippova, Sergey Kovalchuk, Rustam Ziganshin, Anna Mamaeva, Vassili Lazarev, Ivan Latsis, Elena Mikhalchik, Oleg Panasenko, Oleg Ivanov, Vadim Ivanov & Igor Fesenko. Possible role of small secreted peptides (SSPs) in immune signaling in bryophytes. Plant Mol Biol. 2021 May;106(1-2):123-143. doi: 10.1007/s11103-021-01133-z.
  2. Evgeniy G. Evtushenko ,Dmitry V. Bagrov,Vassili N. Lazarev,Mikhail A. Livshits,Elena Khomyakova. Adsorption of extracellular vesicles onto the tube walls during storage in solution. PLoS One (2020)
  3. Ekaterina Grafskaia Elizaveta Pavlova Vladislav V Babenko Ivan Latsis Maja Malakhova Lavrenova Victoria Pavel Bashkirov Dmitrii Belousov Dmitry Klinov Vassili Lazarev. The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides. International Journal of Molecular Sciences (2020) DOI:10.3390/ijms21197141
  4. П.А. Бобровский, В.Д. Мороз, В.Н. Лавренова, В.А. Манувера, В.Н. Лазарев. Ингибирование хламидийной инфекции путем CRISPR/Cas9-SAM опосредованного усиления экспрессии генов пептидогликан-распознающих белков человека в клетках линии HeLa. БИОХИМИЯ (2020) DOI: 10.31857/S0320972520110032
  5. Е. М. Малиновская, Е. А. Кожина, Е. С. Ершова, М. С. Конькова, В. П. Вейко, П. А. Бобровский, В. Н. Лазарев, Л. В. Каменева, Н. Н. Вейко, С. В. Костюк. Исследование роли ГЦ-богатых последовательностей в составе внеклеточной ДНК в активации ДНК-сенсоров раковых клеток на примере линии MCF7. МЕДИЦИНСКАЯ ГЕНЕТИКА (2020)DOI: 10.25557/2073-7998.2020.06.93-95
  6. Dmitriy A Shirokov, Valentin A Manuvera, Olga A Miroshina, Alexandr S Dubovoi, Galina N Samuseva, Margarita E Dmitrieva, Vassili N Lazarev. Generation of recombinant VP3 protein of infectious bursal disease virus in three different expression systems, antigenic analysis of the obtained polypeptides and development of an ELISA test. Arch Virol. 2020 Jul;165(7):1611-1620. doi: 10.1007/s00705-020-04650-2. Epub 2020 May 13.
  7. Vladislav V. Babenko, Oleg V. Podgorny, Valentin A. Manuvera, Artem S. Kasianov, Alexander I. Manolov, Ekaterina N. Grafskaia, Dmitriy A. Shirokov, Alexey S. Kurdyumov, Dmitriy V. Vinogradov, Anastasia S. Nikitina, Sergey I. Kovalchuk, Nickolay A. Anikanov, Ivan O. Butenko, Olga V. Pobeguts, Daria S. Matyushkina, Daria V. Rakitina, Elena S. Kostryukova, Victor G. Zgoda, Isolda P. Baskova, Vladimir M. Trukhan, Mikhail S. Gelfand, Vadim M. Govorun, Helgi B. Schiöth & Vassili N. Lazarev . Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches. BMC Genomics. 2020 Apr 29;21(1):331. doi: 10.1186/s12864-020-6748-0.
  8. P.A. Bobrovsky A.K. Larin N.F. Polina V.N. Lazarev. Transcriptional Analysis of HELA Cells - Producers of the Recombinant Peptidoglycan Recognition Protein PGLYRP1 at Different Stages of the Chlamydia Trachomatis Infection Development. Biomedical Chemistry: Research and Methods (2019) DOI:10.18097/BMCRM00113
  9. Oleg V Podgorny, Nadezhda F Polina, Vassili N Lazarev. Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection. Methods Mol Biol. 2019;2042:137-150. doi: 10.1007/978-1-4939-9694-0_10.
  10. Olga V.Morozova, Olga A.Levchenko, Zlata A.Cherpakova, Valery V.Prokhorov, Nikolay A.Barinov, Ekaterina A.Obraztsova, Aleksandra M.Belova, Kirill A.Prusakov, Konstantin G.Aldarov, Dmitry V.Basmanov, Victoria N.Lavrenova, Elizaveta R.Pavlova, Dmitry V.Bagrov, Vassili N.Lazarev, Dmitry V.Klinov. Surface modification with polyallylamines for adhesion of biopolymers and cells. International Journal of Adhesion and Adhesives (2019)
  11. Iulia I.Pavlova, Vladimir B.Tsvetkov, Ekaterina A.Isaakova, Vyacheslav V.Severov, Ekaterina A.Khomyakova, Ivan A.Lacis, Vassilii N.Lazarev, Maria A.Lagarkova, Galina E.Pozmogova, Anna M.Varizhuk. Transcription-facilitating histone chaperons interact with genomic and synthetic G4 structures. International Journal of Biological Macromolecules (2019)
  12. Liubov A Dadinova , Yurii M Chesnokov 1, Roman A Kamyshinsky, Ivan A Orlov, Maxim V Petoukhov , Andrey A Mozhaev, Ekaterina Yu Soshinskaya, Vassili N Lazarev, Valentin A Manuvera, Anton S Orekhov, Alexander L Vasiliev, Eleonora V Shtykova . Protective Dps-DNA co-crystallization in stressed cells: an in vitro structural study by small-angle X-ray scattering and cryo-electron tomography. FEBS Lett. 2019 Jun;593(12):1360-1371. doi: 10.1002/1873-3468.13439. Epub 2019 May 28.
  13. Белова АМ, Басманов ДВ, Прусаков КА, Лазарев ВН, Клинов ДВ, Микрофлюидная платформа для создания биосенсора на одиночных генетически модифицированных клетках Helicobacter pylori, p 923-932, Биофизика, vol:63 (2019)
    DOI: 10.1134/S0006302918050113
  14. Belova AM, Basmanov D, Babenko VV, Podgorny OV, Mitko T V, Prusakov K, Klinov DV, Lazarev VN, Two novel transcriptional reporter systems for monitoring Helicobacter pylori stress responses, Plasmid volume:106, (2019)
    DOI: 10.1016/j.plasmid.2019.102442
  15. Fesenko, I; Azarkina, R; Kirov, I; Kniazev, A; Filippova, A; Grafskaia, E; Lazarev, V; Zgoda, V; Butenko, I; Bukato, O; Lyapina, I; Nazarenko, D; Elansky, S; Mamaeva, A; Ivanov, V; Govorun, V
    Phytohormone treatment induces generation of cryptic peptides with antimicrobial activity in the Moss Physcomitrella patens
    BMC PLANT BIOLOGY (2019) DOI: 10.1186/s12870-018-1611-z
  16. Fesenko, I; Kirov, I; Kniazev, A; Khazigaleeva, R; Lazarev, V; Kharlampieva, D; Grafskaia, E; Zgoda, V; Butenko, I; Arapidi, G; Mamaeva, A; Ivanov, V; Govorun, V
    Distinct types of short open reading frames are translated in plant cells
    GENOME RESEARCH (2019) DOI: 10.1101/gr.253302.119
  17. Grafskaia, EN; Nadezhdin, KD; Talyzina, IA; Polina, NF; Podgorny, OV; Pavlova, ER; Bashkirov, PV; Kharlampieva, DD; Bobrovsky, PA; Latsis, IA; Manuvera, VA; Babenko, VV; Trukhan, VM; Arseniev, AS; Klinov, DV; Lazarev, VN
    Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2019) DOI: 10.1016/j.ejmech.2019.06.080
  18. Baskova I.P., Kalabushev S.N., Akhaev D.N., Bobrovsky P.A., Manuvera V.A., Lazarev V.N. Role of isopeptidolysis in the process of thrombolysis, Thrombosis Research, Vol 165, 18–23. doi: 10.1016/j.thromres.2018.03.007
  19. Grafskaia E.N., Polina N.F., Babenko V.V., Kharlampieva D.D., Bobrovsky P.A., Manuvera V.A., Farafonova T.E., Anikanov N.A., Lazarev V.N. Discovery of novel antimicrobial peptides: A transcriptomic study of the sea anemone Cnidopus japonicus, Journal of bioinformatics and computational biology  (2018). doi 10.1142/S0219720018400061.
  20. Курдюмов А.С., Манувера В.А., Ахаев Д.Н., Баскова И.П., Лазарев В.Н. Рекомбинантная дестабилаза медицинской пиявки: получение и свойства. // Биоорганическая химия. (2016). Т.42. C.50-61.
  21. Anastasia Nikitina, Vladislav Babenko, Tatyana Akopian, Dmitriy Shirokov, Valentin Manuvera, Alexey Kurdyumov, Elena Kostryukova & Vassili Lazarev, Draft mitochondrial genomes of Hirudo medicinalis and Hirudo verbana (Annelida, Hirudinea), Journal Mitochondrial DNA Part B Volume 1, 2016 - Issue 1
  22. N. Safonova, N. N. Mordkovich, V. P. Veiko, N. A. Okorokova, V. A. Manuvera, P. V. Dorovatovskii, V. O. Popov and K. M. Polyakov, Concerted action of two subunits of the functional dimer of Shewanella oneidensis MR-1 uridine phosphorylase derived from a comparison of the C212S mutant and the wild-type enzyme, Acta Cryst. (2016). D72, 203-210 doi:10.1107/S2059798315024353
  23. Bobrovsky, V. Manuvera, N. Polina, O. Podgorny, K. Prusakov, V. Govorun, V. Lazarev, Recombinant Human Peptidoglycan Recognition Proteins Reveal Antichlamydial Activity, Infection and immunity, 84 (2016) 2124-2130.
  24. Evgeny D. Nekrasov, Vladimir A. Vigont, Sergey A. Klyushnikov, Olga S. Lebedeva, Ekaterina M. Vassina, Alexandra N. Bogomazova, Ilya V. Chestkov, Tatiana A. Semashko, Elena Kiseleva, Lyubov A. Suldina, Pavel A. Bobrovsky, Olga A. Zimina, Maria A. Ryazantseva, Anton Yu. Skopin, Sergey N. Illarioshkin, Elena V. Kaznacheyeva, Maria A. Lagarkova and Sergey L. Kiselev, Manifestation of Huntington’s disease pathology in human induced pluripotent stem cell-derived neurons, Molecular Neurodegeneration201611:27 DOI: 10.1186/s13024-016-0092-5
  25. Podgorny OV, Lazarev VN, Laser microdissection: A promising tool for exploring microorganisms and their interactions with host, J Microbiol Methods. 2016 Jan 13. pii: S0167-7012(16)30001-X. doi: 10.1016/j.mimet.2016.01.001.
  26. Alexey S. Kurdyumov, Valentin A. Manuvera, Isolda P. Baskova and Vassili N. Lazarev, A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein—Destabilase-Lysozyme from medicinal leech, BMC Biochemistry201516:27 DOI: 10.1186/s12858-015-0056-3
  27. Livshits MA, Khomyakova E, Evtushenko EG, Lazarev VN, Kulemin NA, Semina SE, Generozov EV, Govorun VM, Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol, Sci Rep. 2015 Nov 30;5:17319. doi: 10.1038/srep17319.
  28. Lobas AA, Karpov DS, Kopylov AT, Solovyeva EM, Ivanov MV, Ilina IY, Lazarev VN, Kuznetsova KG, Ilgisonis EV, Zgoda VG, Gorshkov MV, Moshkovskii SA, Exome-based proteogenomics of HEK-293 human cell line: Coding genomic variants identified at the level of shotgun proteome, Proteomics. 2016 Jul;16(14):1980-91. doi: 10.1002/pmic.201500349.
  29. Natalia Kalinina, Daria Kharlampieva, Marina Loguinova, Ivan Butenko, Olga Pobeguts, Anastasia Efimenko, Luidmila Ageeva, George Sharonov, Dmitry Ischenko, Dmitry Alekseev, Olga Grigorieva, Veronika Sysoeva, Ksenia Rubina, Vassiliy Lazarev and Vadim Govorun, Characterization of secretomes provides evidence for adipose-derived mesenchymal stromal cells subtypes, Stem Cell Research & Therapy20156:221 DOI: 10.1186/s13287-015-0209-8
  30. Manuvera VA, Kurdyumov AS, Filonova KA, Lazarev VN. Generation of recombinant destabilase-lysozyme from medicinal leeches in three different expression systems. // Protein Expr Purif. (2015) pii: S1046-5928(15)30032-2. doi: 10.1016/j.pep.2015.08.012.
  31. Nikitina AS, Kharlampieva DD, Babenko VV, Shirokov DA, Vakhitova MT, Manolov AI, Shkoporov AN, Taraskina AE, Manuvera VA, Lazarev VN, Kostryukova ES. Complete Genome Sequence of an Enterotoxigenic Bacteroides fragilis Clinical Isolate. //Genome Announc.( 2015). pii: e00450-15. doi: 10.1128/genomeA.00450-15.
  32. Kharlampieva D.D., Manuvera V.A., Podgorny O.V., Grafskaia E.N., Kovalchuk S.I., Pobeguts O.V., Altukhov I.A., Govorun V.M., Lazarev V.N. Recombinant fragilysin isoforms cause E-cadherin cleavage of intact cells and do not cleave isolated E-cadherin. // Microb Pathog (2015). V. 83-84. P. 47-56. doi: 10.1016/j.micpath.2015.05.003
  33. Podgorny O.V., Polina N.F., Babenko V.V., Karpova I.Y., Kostryukova E.S., Govorun V.M., Lazarev V.N.  Isolation of single Chlamydia-infected cells using laser microdissection. // J Microbiol Meth (2015). V.109. P. 123-128.
  34. Kozlov S.A., Lazarev V.N., Kostryukova E.S., Selezneva O.V., Ospanova E.A., Alexeev D.G., Govorun V.M., Grishin E.V. Comprehensive analysis of the venom gland transcriptome of the spider Dolomedes fimbriatus. // Scientific Data (2014). 1:140023. doi: 10.1038/sdata.2014.23
  35. Kharlampieva DD, Manuvera VA, Podgorny OV, Kovalchuk SI, Pobeguts OV, Altukhov IA, Alexeev DG, Lazarev VN, Govorun VM. Purification and characterisation of recombinant Bacteroides fragilis toxin-2. // Biochimie (2013). V.95. P.2123-2131.  doi: 10.1016/j.biochi.2013.08.005
  36. Lazarev VN, Shkarupeta MM, Polina NF, Kostrjukova ES, Vassilevski AA, Kozlov SA, Grishin EV, Govorun VM Antimicrobial peptide from spider venom inhibits Chlamydia trachomatis infection at an early stage. // Arch Microbiol. (2013). V.195. P.173-179. doi: 10.1007/s00203-012-0863-5.
  37. Nadezhda F. Polina, Marina M. Shkarupeta, Anna S. Popenko, Alexander A. Vassilevski, Sergey A. Kozlov, Eugene V. Grishin, Vassili N. Lazarev and Vadim M. Govorun. Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection. // Probiotics & Antimicro. Prot. (2012) V.4. P.208-216. doi: 10.1007/s00203-012-0863-5.
  38. Подгорный О.В., Лазарев В.Н., Говорун В.М. Лазерная микродиссекция в биологии и медицине. // Обзор. Цитология. (2012). Т 54. №5. стр. 381-389.
  39. Alexeev D., Kostrjukova E., Aliper A., Popenko A., Bazaleev N., Tyakht A., Selezneva O., Akopian T., Prichodko E., Kondratov I., Chukin M., Demina I., Galyamina M., Kamashev D., Vanyushkina A., Ladygina V., Levitskii S., Lazarev V., Govorun V. Application of Spiroplasma melliferum Proteogenomic Profiling for the Discovery of Virulence Factors and Pathogenicity Mechanisms in Host-associated Spiroplasma. // J Proteome Res. (2012). V.11. P. 224-236. doi: 10.1021/pr2008626.
  40. Vishnyakov I.E., Levitskii S.A., Manuvera V.A., Lazarev V.N., Ayala J.A., Ivanov V.A., Snigirevskaya E.S., Komissarchik Y.Y., Borchsenius S.N. The identification and characterization of IbpA, a novel α-crystallin-type heat shock protein from mycoplasma. // Cell Stress Chaperones. (2012). V.17. P.171-180. doi: 10.1007/s12192-011-0297-z.
  41. Lazarev V.N., Polina N.F., Shkarupeta M.M., Kostrjukova E.S., Vassilevski A.A., Kozlov S.A., Grishin E.V., Govorun V.M. Spider Venom Peptides for Gene Therapy of Chlamydia Infection. // Antimicrob Agents Chemother. (2011). V.55. P. 5367–5369.  doi: 10.1128/AAC.00449-11.
  42. Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A., Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I., Alexeev D.G., Ladygina V.G., Govorun V.M. Complete Genome and Proteome of Acholeplasma laidlawii. // J Bacteriol. (2011). V.193. P. 4943-4953. 10.1128/JB.05059-11.
  43. Levitskiy S.A., Sycheva A.M., Kharlampieva D.D., Oberto J., Kamashev D.E., Serebryakova M.V., Moshkovskii S.A., Lazarev V.N., Govorun V.M. Purification and functional analysis of recombinant Acholeplasma laidlawii histone-like HU protein. // Biochimie (2011). V.93. P.1102-1109. doi: 10.1016/j.biochi.2011.03.005.
  44. Lazarev V.N., Borisenko G.G., Shkarupeta M.M., Demina I.A., Serebryakova M.V., Galyamina M.A., Levitskiy S.A., Govorun V.M. The role of intracellular glutathione in the progression of Chlamydia trachomatis infection. // Free Radic Biol Med. (2010). V.49. P.1947-1955. doi: 10.1016/j.freeradbiomed.2010.09.024
  45. Lazarev VN, Govorun VM. Antimicrobial peptides and their use in medicine. // Appl Biochem Microbiol (2010).V.46. P.803–814 doi: 10.1134/S0003683810090012
  46. Л.Л. Завалова, В.Н. Лазарев,С.А. Левицкий, Т.Г. Юдина, И.П. Баскова.  Дестабилаза-лизоцим медицинской пиявки. Полифункциональность рекомбинантного белка. // Биохимия. (2010). V.75. №9. стр. 1314-1324. doi: 10.1134/S0006297910090129
  47. Вишняков И. Е., Левицкий С. А., Лазарев В. Н., Айала X. А., Иванов В. А., Снигиревская Е. С, Комиссарчик Я. Ю., Борхсениус С. Н. 2010. Олигомерные формы, функции и локализация в клетке белка α-кристаллинового типа из микоплазмы. // Цитология (2010). № 52. стр. 938–945.